摘要
绒毛状烟草是重要的野生种烟草之一。本研究以CopyControlTMpCClBACTM（HindⅢCloning-Ready）Vector为载体，构建了绒毛状烟草的BAC文库。分析结果表明，BAC文库DNA插入片段为50-200kb，平均110kb，空载率〈2％。随机挑选15个克隆进行稳定性继代试验，100代后插入片段仍然稳定存在。该绒毛状烟草BAC文库的建成，为进一步克隆重要功能基因提供了必备的物质平台。
Villi tobacco （Nicotiana tomentosiformis） is an important species of wild tobacco. The BAC library was constructed with CopyControlTM pCC1BACTM （HindIII Cloning-Ready） Vector. From the analysis of randomly selected BAC clones, the inserted DNA size of BAC library ranged from 50 to 200 kb; the average estimated was 1 l0 kb; the empty clone was less than 2%; the insert was stable in a hundred generations.
出处
《中国烟草科学》
CSCD
2012年第3期68-71,共4页
Chinese Tobacco Science
基金
国家烟草专卖局重点科技项目“烟草cDNA文库构建及重要基因表达谱研究”(110200701021)
