摘要
目的研究多种中药对于脑淀粉样血管病相关蛋白胱抑素聚集的影响。方法通过在胱抑素酵母表达系统的诱导培养基中添加亚适量浓度中药成分的方式,应用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)技术结合蛋白质免疫印迹(Western blotting)技术,在胞外和胞内检测中药对鸡胱抑素蛋白聚集的影响。结果胞外的药物筛选实验表明远志对于胱抑素的聚集有明显的抑制作用,而且远志的浓度在500μg/mL以下时,药物剂量与抑制胱抑素聚集效果呈现较好的正相关性;胞内实验表明远志对细胞内胱抑素聚集没有影响。结论远志对于分泌至胞外的胱抑素聚集有明显的抑制作用。
Objective The primary aim of this study is to investigate the effect of traditional Chinese medicine on the aggregation of Human cystatin C,a kind of cerebral amyloid disease-related protein.Methods With the addition of suboptimal concentrations of Chinese medicine to the induction medium of cystatin yeast expression system,the effects of traditional Chinese medicine on cystatin is determined both in vitro and in vivo using SDS-PAGE and western blotting.Results Extracellular experiments demonstrated that Polygala significantly represses the aggregation of cystatin.The concentration of Polygala has a positive relationship to its inhibitory effect on cystatin aggregation at the concentration below 500 μg/mL.Intracellular experiments showed that Polygala has no effect on cystatin aggregation in vivo.Conclusion Polygala has an obvious inhibitory effect on the aggregation of secreted cystatin.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第20期2009-2011,2014,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(30970152)
辽宁省教育厅优秀人才项目(2009R26)
教育部留学回国人员科研启动基金资助项目(教外司留[2010]1561号)
关键词
脑淀粉样血管病
远志
胱抑素
蛋白聚集
cerebral amyloid angiopathy
polygala tenuifolia
cystatin
protein aggregation