康复训练对脑出血大鼠室管膜下区NSCs增殖与分化的影响

Effects of Rehabilitationtraining on the Neural Stem Cells Proliferation and Differentiation in SVZ of Rats With Intracerebral Hemorrhage Stroke

目的探讨康复训练对大鼠脑出血后室管膜下区(SVZ)神经干细胞(NSCs)增殖和分化的影响。方法 75只SD大鼠随机分为康复组、制动组和假手术组(每组25只),康复组和制动组用胶原酶诱导脑出血模型,假手术组用生理盐水替代胶原酶。康复组每天予以抓握、平衡、旋转等训练,制动组置于网状笼内固定,假手术组在笼内自由活动。每组大鼠康复前后进行神经功能评分,分别于康复训练后1、4、7、14和28天处死动物。应用免疫组织化学方法检测大鼠SVZ神经细胞巢蛋白(Nestin)和5-溴脱氧尿核苷(BrdU)表达的变化。结果康复组神经功能缺损评分低于制动组(P<0.05)。康复组大鼠SVZ BrdU阳性、Nestin阳性细胞在不同时间点均多于制动组大鼠(P<0.05),第7天康复组SVZ BrdU阳性、Nestin阳性细胞表达达到高峰,14天时表达逐渐减少(P<0.05,P<0.01)。结论康复训练可促进脑出血后大鼠NSCs增殖,并向神经元分化。

Objective To investigate the influence on the neural stem cell proliferation and differentiation by rehabilitation training after the rat suffering cerebral hemorrhage. Methods 75 SD rats were randomly divided into rehabilitation group, braking group and sham-operation group （25 in each group） , the rehabilitation group and the braking group had collagenase-induced intracerebral hemorrhage, the sham-operation group had normal saline instead of collagenase. The rats of rehabilitation group had the daily training of grasping, balance, rotation and so on, the braking group rats were arranged in the mesh cage, then the neurological function was scored before and after the rats of each group making their recovery, later the animals were sacrificed respectively after the rehabilitation training of 1 day, 4 days, 7 days, 14 days, and 28 days. Immunohistochemistry is used to detect it and then the result is expressed by Nestin, BrdU. Results The neural function defect score of the rehabilitation group is lower than the braking group, the two groups have significant difference （ P 〈 0. 05 ） ; at different time the SVZ BrdU and Nestin cells of rehabilitation group are more than the braking group （ P 〈 0. 05 ）, at the first 7 days, in the rehabilitation group SVZ area BrdU , Nestin cells expression reached the peak, at 14d the expression gradually decreased. Conclusions Rehabilitation training can promote the proliferation of neural stem cells after cerebral hemorrhage, and the differentiation into neurons.