中国广州汉族人群六个STR位点的调查

Chinese Han population data obtained from fluorescently detected STR loci vWA31A,TH01,F13A01,FES,TPOX and CSF1PO

目的 了解广州汉族人在vWA31A等6个短串联重复序列(shorttandemrepeat,STR)位点法医学的有关数据。方法 运用STRPCR、4%变性聚丙烯酰胺凝胶电泳,结合荧光DNA自动检测技术,对汉族人群6个STR位点的等位基因频率和基因型频率进行了调查,并与其他种族或人群的等位基因频率进行了比较。结果 6个STR位点是vWA31A、TH01、F13A01、FES、TPOX和CSF1PO,所有位点的结果均符合HardyWeinberg平衡,且各位点等位基因间无相关性,除vWA31A外,其它5个位点的杂合度均低于白种人和黑种人。6个位点的累积DP=099999,PE=09708,pM=1059×105。结论 所得到的等位基因频率等数据可为中国汉族人群法医个人识别、亲子鉴定及遗传学研究提供依据。

Objective This paper was designed to evaluate 6 short tandem repeats(STR) loci for their use in forensic personal identification.Methods The allele frequencies and genotype frequencies at 6 tetranucleotide STR loci were determined in a sample of 132 unrelated individuals from a Chinese population of Han nationality in Guangzhou.The PCR products were analysed on 4% denaturing PAGE and detected using fluorescently labelled primers in an automated 377 sequencer(PE). The resultant allele frequencies were compared with other population or race databases.Results All the 6 loci (vWA31A,TH01,F13A01,FES, TPOX,CSF1PO)met Hardy Weinberg equilibrium.No random association of alleles among the 6 loci was noted. Except locus vWA31A, the observed heterozygosities at other 5 loci were significantly lower than those reported in Caucasian and Black population studied. The calculated DP=0 99999,PE=0 9708,pM=1 059×10 5 .Conclusion The data obtained can be used in human identity and paternity testing, and in other genetic researches and population investigation.