应用高迁移率族蛋白B1与单核细胞上Toll样受体4探索系统性红斑狼疮的病情活动

Exploring the pathogenesis of the systemic lupus erythematosus by detecting Toll like receptor 4 and high mobili-ty group box chromosomal protein 1

目的通过检测系统性红斑狼疮(SLE)患者血清中高迁移率族蛋白B1(HMGB1)及其外周血单核细胞上Toll样受体4(TLR4)的表达,评价其在SLE病情进展中的作用。方法随机选取SLE患者40例,活动期与稳定期各20例,健康对照组20名,采用酶联免疫吸附试验(ELISA)法检测血清中HMGB1蛋白的表达,采用流式细胞术(FCM)分析外周血单核细胞上TLR4的表达。统计学分析其与SLE临床及实验室指标间的相关性。结果 SLE活动组血清HMGB1表达水平较稳定组SLE和健康对照组明显增高(P<0.05),而SLE稳定组和健康对照组之间血清HMGB1表达水平差异无统计学意义(P>0.05);活动组SLE患者外周血单核细胞TLR4的表达较稳定组SLE和健康对照组相比呈上调趋势,而TLR4的表达模式在SLE稳定组和健康对照组之间差异无统计学意义;血清中HMGB1的表达水平与单核细胞表面TLR4的表达呈正相关。结论血清HMGB1和外周血单核细胞表面TLR4均参与了SLE的病情发展过程,且其在SLE发病机制中可能具有协同作用。

Objective To evaluate the pathogenesis and the role of disease progression in systemic lupus erythem- atosus （SLE） by detecting the expression of high mobility group box chromosomal protein 1 （HMGB1） in serum, Toll like receptor （TLR） 4 in monocyte derived macrophage in systemic lupus erythematosus patients. Methods Forty patients with SLE were selected randomly, of which 20 patients were activity groups and the others were stable groups. The expres- sion of HMGB1 in serum of these cases were detected by enzyme-linked immunosorbent assay （ELISA）; the expression of TLR4 on CD14~ monocytes in peripheral blood were detected by flow cytometry （FCM）; the correlation between these indexes and clinical, laboratory indexes about SLE was analyzed by statistical methods. Results The expression levels of HMGB1 in serum of the active SLE group compared with the stable and normal control group was significantly higher （P〈 0.05）. But there was no significant difference of the serum HMGB1 expression levels between the stable SLE and normal control group （P〉0.05）. The expression of TLR4 in peripheral blood mononuclear cells derived macrophages in the active SLE group compared with the stable and normal control group was up-regulated, but there was no significant difference between the expression of TLR4 of the stable and that of normal control group （P〉0.05）. There were positive correlation between the serum levels of HMGB1 and TLR4 in peripheral blood mononuclear cells derived macrophages （P〈0.05）. Conclusion The expression levels of HMGB1 in serum and the expression of TLR4 in peripheral blood mononuclear cells derived macrophages are participated in the pathological processes of SLE, and there may be synergies in the pathogenesis of SLE.