摘要
目的了解脂肪酸气相色谱分析方法能否应用于铜绿假单胞菌的同源性分析。方法收集某院2008年1—12月重症监护室住院患者痰标本分离的26株多重耐药铜绿假单胞菌,分别应用脉冲场凝胶电泳(PFGE)和脂肪酸气相色谱分析方法进行同源性分析,比较两组结果。结果 PFGE结果表明,26株菌有6个克隆;脂肪酸气相色谱分析显示,26株菌存在4个克隆,符合率仅26.92%(7/26)。结论脂肪酸气相色谱分析方法在菌株同源性判断的准确性上与PFGE分型有差距,其应用于铜绿假单胞菌的同源性分析仍需谨慎。
Objective To investigate whether gas chromatographic whole-cell fatty acid analysis can be applied to analyze the homology of Pseudomonas aeruginosa. Methods Twenty-six multidrug-resistant Pseudomonas aerugi- nosa （MDR-PA） strains isolated from sputum samples of intensive care unit （ICU） patients were collected from Jan- uary to December 2008. Pulsed-field gel electrophoresis （PFGE） and gas chromatographic whole-cell fatty acid anal- ysis were performed to analyze the homology. Results PFGE analysis revealed that 26 isolates had 6 clones; gas chromatographic whole-cell fatty acid analysis showed that 26 strains had 4 clones, and consistent rate was only 26. 92% （7/26）. Conclusion Gas chromatographic whole-cell fatty acid analysis is not identical with PFGE in analy- zing homology of Pseudomonas aeruginosa.
出处
《中国感染控制杂志》
CAS
2012年第3期161-164,共4页
Chinese Journal of Infection Control
基金
广州市教育局创新团队科研基金(B94117)
广州市科技计划项目(2010J-E171)
关键词
铜绿假单胞菌
脉冲场凝胶电泳
气相色谱分析
同源性
实验室技术与方法
Pseudomonas aeruginosa
pulsed-field gel electrophoresisl gas chromatographic analysis
homology
laboratory technique and method
