斑蝥素与去甲斑蝥素对昆虫细胞Sf9抑制作用比较

Comparisons of inhibition between cantharidin and norcantharidin on Sf9 cells

为探索斑蝥素的类似物去甲斑蝥素对昆虫的毒杀机理,本研究利用草地贪夜蛾Spodoptera frugiperda卵巢细胞系Sf9细胞作为实验材料,采用CCK-8法和AO/EB荧光染色法比较了斑蝥素(CTD)和去甲斑蝥素(NCTD)对细胞的增殖抑制和抑制方式。结果显示,3.125μg/mL的CTD处理细胞抑制率6h为20.00±1.10%,24h为66.33±0.81%;50μg/mL的CTD处理细胞抑制率6h为42.69±6.34%,24h为74.60±1.51%。3.125μg/mL的NCTD处理细胞抑制率6h为10.03±1.72%,24h为21.75±6.22%;50μg/mL的NCTD处理细胞抑制率6h为40.79±1.32%,24h为66.08±3.32%。结果表明,CTD与NCTD均能有效抑制Sf9细胞增殖和诱导细胞凋亡,且抑制率呈现出时间、剂量依赖性,较高剂量NCTD可以替代CTD达到相同效果。

To investigate the mechanism of NCTD＇s toxic effect on Sf9 cells,CCK-8 assay and AO/EB fluorescent dyes were used to detect the inhibition rates and the way of function.There results showed that,treated with CTD of 3.125 g/mL after 6 hour and 24 hour,the inhibition rates of test cells were 20.00 ± 1.10% and 66.33 ± 0.81% ;treated with CTD of 50 g/mL after 6 hour and 24 hour,the inhibition rates of test cells were 42.69 ± 6.34% and 74.60 ± 1.51%.Treated with NCTD of 3.125 g/mL after 6 hour and 24 hour,the inhibition rates of test cells were 10.03 ± 1.72% and 21.75 ± 6.22% ;treated with NCTD of 50 g/mL after 6 hour and 24 hour,the inhibition rates of test cells were 40.79 ± 1.32% and 66.08 ± 3.32%.It was indicated that,both of CTD and NCTD could effectively inhibit cell＇s proliferation in concentration-dependent and time-dependent,higher concentration of NCTD could replace lower concentration of CTD to reach similar inhibition rates,and NCTD could induce apoptosis significantly as well as CTD.