百色市农村散养犬狂犬病免疫抗体消长情况调查报告

The Investigation of Immune antibodies of domestic dogs in Countries in Baise City

为了进一步了解不同种类的狂犬病疫苗对农村散养犬免疫效果,对百色市的6个县所辖的12个自然屯的散养犬分别使用国产兽用狂犬病活疫苗(Flury株)和进口兽用狂犬病灭活疫苗(G52株)以不同方式免疫。在免疫前和免疫后7～10d、20～30d、150～180d、340～360d分别采集犬只血清,采用间接酶联免疫法(ELISA)检测抗体水平。结果,未免疫前、使用活疫苗免疫一次、灭活苗免疫一次、活疫苗二次免疫、活疫苗一免后用免疫灭活疫苗再加强免疫一次,其抗体平均阳性率分别为0、70.3%、89.8%、95%、98.8%。结果表明犬只免疫两次以上狂犬病疫苗取得良好的免疫效果,同时进口狂犬病灭活疫苗免疫效果优于国产狂犬病活疫苗。

To learn about immune effect of the different types of rabies vaccines in rural domestic dogs,the dogs in12 villages were immuned with homemade live rabies vaccine（strain Flury） and imported inactivated rabies vaccine（G52） in different ways in 6 county of Baise City.Sera of dogs were collected to detect antibody levels of dogs rabies virus in front of the immune and after immunization about 7-10d,20-30d,150-180d,340-360d respectively with indirect enzyme-linked immunosorbent assay（ELISA） techquieIt was showed that the average positive rate of antibodies were 0,70.3%,89.8%,95%and 98.8% with using a live vaccine and inactivated vaccine only one time,a live vaccine twice and,first time using a live vaccine and second time with inactivated vaccine immune respectively.Results showed that the immune immune effect of dogs rabies vaccine were better with twice than only one time.A nd immune effect of imported inactivated rabies vaccine was better than that of homemade rabies vaccines.