深圳市献血人群乙肝病毒感染调查分析

INVESTIGATION ON HEPATITIS B VIRUS INFECTION OF BLOOD DONORS IN SHENZHEN

目的调查深圳献血人群乙肝病毒感染情况,有效地提高血源质量,降低输血风险。方法采用进口和国产试剂ELISA进行20 400人份血液进行筛查,中和实验及分子生物学PCR方法对阳性结果进行确证分析,阴性标本进一步核酸检测。结果深圳市献血人群HBsAg阳性率在0.55%,HBsAg(-)HBV DNA(+)的比率为1︰5 100。在ELISA阳性146例中,确证阳性112例。进口试剂检出率较国产试剂高。结论为减少输血后乙肝感染,血液筛查有必要采用高灵敏度特异性较好的进口试剂检测HBsAg,进一步应用核酸检测技术检测HBV DNA,提高输血安全。

Objective To investigate the Hepatitis B virus infection condition in Shenzhen Blood donors,raise blood safety and cut down transfuses blood risk.Methods Two-round blood screening for 20 400 blood samples were adopted by foreign and domestic ELISA Kits,and all positive samples were confirmed with neutralizing tests and PCR method.Results The positive rate of HBsAg in Shenzhen blood donors was 0.55%,and HBsAg（-） HBV DNA（＋） ratio for 1：5100,112 of 146 HBsAg（＋） samples were confirmed positive.The positive rate of foreign kits was higher than domestic kits.Conclusion It is necessary to use for higher-sensitivity and specificity foreign kits to reduce the HBV infection of blood transfusion,and apply nucleic acid tests for further screening to raise blood transfusion safety.