HPLC法测定复方板蓝根口服液中表告依春的含量

HPLC for Determining the Content of Epigoitrin in Compound Banlangen Oral Solution

建立了高效液相色谱法测定复方板蓝根口服液中表告依春的含量。采用高效液相色谱法。色谱柱为Aigleng C18柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.01 mol/L磷酸二氢钠缓冲液(氢氧化钠试液调节pH 7.2)(7∶93)流速:1.0 mL/min,检测波长240 nm;柱温:30℃。结果显示:表告依春色谱峰分离良好,表告依春进样量在0.01938～0.3876μg范围内线性关系良好,回归方程:A=7154.3C-22.73,r=0.9999;平均回收率为99.7%,RSD为0.77%(n=6)。该测定方法简便,准确,重现性好,精度高,可用于该制剂的质量控制。

HPLC for determining the content of Epigoitrin in Compound Banlangen Oral Solution was established. Stationary phase was Aigleng C18 chromatographic column （4.6 ×250mm, 5μm）, mobile phase was methanol -0.01 mol/L sodium dihydrogen phosphate buffer solution （ Adijusted to pH 7.2 with sodium hydroxide solution） （ 7 ： 93 ）, de- tection wavelength was 240 nm, flow rate was 1.0 mL/min and column temperature was 30℃. Epigoitrin had a good separation of peaks, the calibration curve were linear over the range of 0.01938 -0.3876 μg, and the regression equation was A = 7154.3C - 22.73, r = 0.9999. The average recovery was 99.7% with RSD = 0.77% （n = 6）. This method was simple, accurate, with good reproducibility and high precision, and can be used to control the quality of Compound Ban- langen Oral Solution.