檀香种子生活力快速测定法

Study on methods for rapid determination of seed viability of Santalum album

采用四唑染色法、靛蓝染色法以及碘化钾反应法(I-KI)对檀香(Santalum album)种子生活力进行测定,分析了四唑的质量分数、染色温度以及染色时间对檀香种子生活力测定的影响,并将各种不同生活力测定方法的测定结果与发芽试验结果进行了比较分析。结果表明,四唑法测定檀香种子生活力的最佳测定条件为使用质量分数为0.1%的四唑,在40℃染色3 h。四唑的质量分数(p<0.001)、染色温度(p=0.006)以及质量分数和染色温度之间的交互作用(p=0.001)对檀香种子生活力的测定结果均有显著影响。不同的生活力测定方法之间差异显著(p<0.001),其中以四唑法测定结果与发芽率接近,是檀香种子生活力快速测定的理想方法。靛蓝染色法和碘化钾反应法均不适合用作檀香种子生活力的快速测定。

In order to find the most suitable method for rapid determination of seed viability of sandal （Santalum al- bum）, TTC method, indigo dying method as well as I - KI reaction method were studied by comparing with the germina- tion test. The results showed that the best testing condition for sandal seed viability determination was staining 3 h with 0.1% TTC at 40 ℃ under darkness. Significant differences were found among TI＇C concentration （p 〈 0. 001 ）, stai- ning temperature （p = 0.006 ） and the interaction between TTC concentration and staining temperature （p = 0. 001 ）, great differences also existed among different testing methods （p 〈 0. 001 ）. TTC method got the most similar results with the germination rate, and would be the most suitable method for rapid determination of seed viability of sandal, while in- digo dying method and I -KI reaction method were not suitable.