摘要
目的探讨TNFα-863 C/A基因型和基因变异与葡萄膜炎发病的可能关系。方法使用多聚酶链反应(PCR-RFLP)技术检测51例葡萄膜炎及121例正常人肿瘤坏死因子TNFα-863 C/A基因多态性。结果葡萄膜炎组肿瘤坏死因子TNFα-863 C/C、A/C、A/A表型频率分别为:0.7255、0.2745、0,对照组分别为0.7521、0.2397、0.0082;葡萄膜炎组TNFα-863 A、TNFα-863 C基因频率分别为:0.8719、0.1281,对照组分别为:0.8627、0.1373;TNFα-863三种基因型频率和基因频率在葡萄膜炎组与正常对照组间无统计学差别(p>0.05)。结论肿瘤坏死因子TNFα-863 C/A基因多态性与葡萄膜炎无明显相关。
Objective To study the relationship between the polymorphism of tumor necrosis factor -α （ TNF - α） - 863 C/A and uveitis in patients. Methods The polymorphism of TNF - α - 863 C/A was detected by poiymerase chain reaction -restriction fragment length polymorphism （PCR -RFLP） method in 51 uveitis patients and 121 healthy controls. Results The gene frequency of TNFα-863 C/A in uveitis group was as followed; TNFα-863 C/C, A/C, A/A genotype frequency ： 0. 7255, 0. 2745,0, TNFα-863 C, TNFα-863 A Gene frequency： 0. 8719,0. 1281. TNFα-863 C/C ,A/C,A/A genotype frequency in control group was as followed： 0. 7521,0. 2398,0. 0082 TNFct -863 C ,TNFα-863 A Gene frequency： 0. 8627,0. 1373. There were no significant differences in the frequencies of TNFα-863 C/A gene between uveitis patients and control group （p 〉 0. 05 ）. Conclusion TNF - α-863 C/A polymorphism was no associated with uveitis and allele may not be a risk factor for uveitis.
出处
《现代医院》
2012年第7期8-10,共3页
Modern Hospitals
基金
番禺区科技计划项目(编号:9-z-83-1)
