钟冠唇柱苣苔组织培养研究

Study on Tissue Culture of Chirita swinglei

为了筛选出适合钟冠唇柱苣苔[Chirita swinglei(Merr.)W.T.Wang]不定芽诱导、分化和生根的培养体系,以其嫩叶为材料,在不同培养条件下进行了组织培养研究。结果表明,用体积分数为75%的乙醇灭菌20 s,再用1 g/L HgCl2浸泡7 min为最佳的外植体灭菌方法;MS+6-BA 0.5 mg/L+NAA 0.1 mg/L为不定芽诱导的最适培养基;MS+6-BA 0.3 mg/L+NAA 0.1 mg/L为不定芽分化的最适培养基;1/2MS+NAA 0.1 mg/L+活性炭1 g/L为最适的生根培养基。

To select a suitable culture system for the adventitious bud induction,differentiation and rooting of Chirita swinglei（Merr.） W.T.Wang,using tender leaves as explants,tissue culture techniques of C.swinglei were studied.The results showed that the best sterilization method was sterilizing by 1 g/L HgCl2 for 7 min after treating with 75% alcohol for 20 s.The optimal medium for inducing adventitious bud was MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L;the optimal medium for adventitious bud differentiation was MS＋6-BA 0.3 mg/L＋NAA 0.1 mg/L;and the optimal medium for rooting culture was 1/2MS＋NAA 0.1 mg/L＋ active carbon 1 g/L.