T细胞Trail受体表达及Trail诱导的T细胞凋亡

The expression of Trail receptor on human T cells and apoptosis induced by Trail

目的 :观察人T细胞Trail(TNF relatedapoptosis inducingligand)受体表达及Trail诱导T细胞凋亡的作用。方法 :通过荧光双标记 ,使用流式细胞仪检测Trail受体阳性细胞和凋亡细胞。结果 :静息Jurkat细胞表达高水平Trail受体〔(49 19±12 2 5 ) %〕 ,激活Jurkat细胞对Trail受体表达无影响。Trail诱导显著的Jurkat细胞凋亡〔(90 0 1± 2 6 71) %〕。正常人外周血淋巴细胞 (PBL)仅激活后表达Trail受体〔(2 5 2 7± 6 42 ) %〕 ,但无细胞凋亡发生。结论 :人T细胞表达Trail受体 ,Trail受体和Trail的相互作用对细胞凋亡有调控作用。

Objective: To investigate the expression of Trail(TNF related apoptosis inducing ligand)receptor on human T cells and apoptosis induced by Trail.Methods: The positive cells for Trail receptor and apoptotic cells were determined by flow cytometry. Results:Trail receptor on unstimulated Jurkat cells was found in (49 19±12 25)% and the stimulation could not increase the expression of Trail receptor on Jurkat cells.The apparent apoptosis of Jurkat cells was found in (90 01±26 71)%. Peripheral blood lymphocytes(PBL)from healthy donors may express Trail receptor after activation (25 27±6 42)%,but no apoptosis was observed in these PBL.Conclusion: Human T cells may express Trail receptor.The ligation of Trail receptor and Trail plays an important role in regulation of apoptosis.