中国人群中一个新的HLA-DRB4等位基因(DRB4*NC)的发现及比较分析

A new factor of HLA class Ⅱ DRB4 alleles in a Chinese individual

目的 :对人群进行HLA DRB的分型及多态性研究 ,发现未曾报道过的HLA DRB等位基因。方法 :用HLA DRB通用引物扩增该个体所有DRB等位基因的第二外显子 ,并对扩增产物进行克隆 ,通过双脱氧法进行DNA测序。结果 :发现一个新的HLA DRB核苷酸序列。结论 :通过与已发表的序列及Genbank已收载的序列比较 ,显示该序列属于HLA DRB4家族的新等位基因 ,我们暂时称它为DRB4 NC。该序列已被Genbank收载 (登录号为AF2 0 75 48)。

Objective:To study on HLA DRB typing and polymorphism and find new HLA DRB allele.Methods:The second exon of all the DRB alleles were amplified by using the HLA DRB generic primers.The PCR products were cloned and sequenced by dideoxy method.Results:A new nucleotide sequence that was highly homologous with HLA DRB4 alleles was found.Conclusion:Through sequence comparing it was sure that the sequece is a novel HLA DRB4 allele.It was named DRB4*NC temporarily to mean the new allele was identified in a Chinese individual.The information of this sequence can be retrieved from the Genbank and its accession number is AF207548.