石油烃对虾夷马粪海胆胚胎发育及四种酶活力的影响

Effect of petroleum hydrocarbons on strongylocentyotus internedius embryonic development and activity of four enzymes

通过虾夷马粪海胆胚胎暴露实验,研究了常见石油烃零号柴油对海胆胚胎发育时间以及发育时期内海胆胚胎的SOD、CAT、GPx及谷胱甘肽转移酶(GST)四种酶活性的影响。结果表明,不同浓度的零号柴油水溶组分对海胆胚胎发育的各个时期具有不同程度的延迟效应,低浓度对海胆胚胎中的四种酶表现出不同程度的诱导效应;四种酶活性的变化程度比较,对GPx和GST酶活力的影响要明显大于对CAT和SOD的影响;随着浓度的不断增大,四种酶活性受到不同程度的抑制作用,高浓度对酶活性的抑制作用尤为显著;囊胚期对影响最为敏感,可作为生物标志物。

0# diesel is a common petroleum hydrocarbon pollutant in sea and marine organisms. The effect of water-accommodated fractions （WAFs） on sea urchin embryo development time and activity of SOD, CAT, GPx and GST in different developmental stages were studied. The results show that different concentrations 0 # diesel oil WAFs have different degrees of delayed effect in different de- velopment periods. Low concentrations WAFs have induction effect on four enzymes of sea urchin embryos. WAFs have Greater impact on GPx and GST than CAT and SOD. With the increasing concentration, four kinds of enzyme activity are inhibited in unequal de- grees. However, the high concentrations inhibited the enzyme activity were particularly significant. The Blastoeyst stage is most sensi- tive to the effects and can be used as biomarkers.