bcl-2,c-myc和p53在大肠腺瘤和大肠癌中的表达

Expression of bcl 2,c myc and p53 in Colorectal Adenoma and Carcinoma

目的 探讨癌基因bcl 2 ,c myc和 p5 3在大肠腺瘤与大肠癌中的表达情况及与临床病理特征的关系。 方法 采用免疫组化ABC法 ,检测 63例大肠癌和 40例大肠腺瘤的bcl 2 ,c myc和 p5 3表达。 结果 bcl 2 ,c myc和 p5 3在大肠腺瘤中的表达分别为 72 .7% ( 2 4/3 3 )、62 .2 % ( 2 3 /3 7)和 45 .0 % ( 18/4 0 ) ,而在大肠癌中的表达则依次为 3 4 .0 % ( 16/4 7)、82 .1% ( 3 2 /3 9)和 84.1% ( 5 3 /63 )。三个癌基因在大肠腺瘤和大肠癌中的表达均有显著的统计学意义 (P <0 .0 1) ,但三个癌基因的表达与临床病理特征无显著的相关性。结论 bcl 2基因的异常激活在肿瘤的发展和演变出现较早 ,而c myc和p5 3的表达则较迟 ,且两者起协同作用。

Objective To investigate the expressions of oncogenes bcl 2,c myc and p53 in colorectal adenoma and adenocarcinoma,and the relationship between the expression of oncogene and clinical pathological features.Method Expression of oncogene bcl 2 were determinated in 33 cases of colorectal adenomas and 47 cases of colorectal carcinomas by the immunohistochemical method(ABC method).Expression of p53 in 40 cases of colotectal adenomas and 63 cases of colorectal carcinomas,in addition to c myc protein in 37 cases of adenoma and 39 cases of colorectal adenocarcinomas were also determinated.Results The positive expression rates of bcl 2 in colorectal adenoma and adenocarcinoma were 72.2%(24/33) and 24.2%(16/47) respectively,and those of p53 were 45.0%(18/40) and 84.1%(53/63) respectively.The Positive staining rates of c myc in colorectal adenoma and adenocarcinoma were 62.7%(23/37) and 82.1%(30/39) respectively.There were significant differences between the expressions of these three oncogenes in colorectal adenoma and in adenocarcinoma( P <0.01).However,the expression of these three oncogenes were not correlated with clinical pathological features.Conclusions The abnormal activation in bcl 2 takes place earlier during the development and progression of colorectal adenocarcinoma.But c myc and p53 express later and cooperate with each other in progression of colorectal adenocarcinoma.