摘要
目的:对续断指纹图谱进行成分归属研究,为科学评价和有效控制其质量提供依据。方法:采用HPLC-TOF/MS技术,通过正离子模式和负离子模式检测获得精确分子量,结合文献和对照品推测续断HPLC-TOF/MS指纹图谱中的化学组成。结果:采用HPLC-TOF/MS法推测了15个成分,用对照品确认了木通皂苷D、绿原酸、咖啡酸、马钱苷、川续断皂苷V、川续断皂苷X、川续断皂苷VII和3-O-L-吡喃阿拉伯糖-常春藤皂苷元等8个成分。结论:该研究比较全面地阐明了续断的化学组成,使指纹图谱的特征性更强,为续断的鉴别与质量评定奠定了基础。
Objective： Research was performed on HPLC -TOF/MS fingerprint to identify compounds in Dipsaeus asperoides. Method： The accurate mass was obtained by HPLC -TOF/MS in both positive and negative ion modes. Combined with literatures components were concluded in D. asperoides by chemical substances. Results： Fifteen components were concluded and eight （ehlorogenic acid, asperosaponin VI, loganin, akebia saponin X, akebia saponin VII, akebia saponin V and 3 - O - a- L - arabinopyranosyl -hederagenin） were identified by comparing with standard substances. Conclusion： This study gave more details of chemical constituents of D. asperoides, which made fingerprint more characteristic. HPLC -TOF/MS technique could be used in quality control of D. asperoides.
出处
《中国野生植物资源》
2012年第3期22-25,共4页
Chinese Wild Plant Resources
基金
国家自然科学基金重点课题(中药盐制与咸入肾
肾主骨相关性研究
30730113)
江苏省高校优势学科建设工程资助项目
