白腹锦鸡和草原雕肝脏结构的光镜观察及Bax蛋白、c-Fos、TGF-β_1的表达

Light microscopical observation and expression of Bax protein, c-Fos,and TGF-β_1 in liver of Chrysolophus amherstiae and Aquila rapax

为了研究动物肝脏结构与机能的关系,用光镜观察了白腹锦鸡(Chrysolophus amherstiae)和草原雕(Aquilarapax)肝脏的显微结构,用免疫组织化学方法检测了肝脏中Bax蛋白、c-Fos、TGF-β1的表达.结果表明,白腹锦鸡肝小叶的肝细胞排列较为规则,肝细胞索呈双行或多行的索状围绕中央静脉呈放射状排列;门管区的小叶间动脉、小叶间静脉和小叶间胆管轮廓明显;肝细胞膜、细胞质呈Bax蛋白、c-Fos、TGF-β1免疫反应阳性.草原雕的肝小叶不规则且分界模糊;肝细胞膜、细胞质等呈Bax蛋白、c-Fos、TGF-β1免疫反应阳性.在两种鸟肝组织中Bax蛋白、c-Fos、TGF-β1的表达强度有差异,三者的共同作用可能调控鸟肝细胞的生长、发育.

Histological structure is observed by light microscopy and expression of Bax protein,c-Fos,TGF-β1 is examined by immunohistochemical methods in Chrysolophus amherstiae and Aquila rapax liver.Results show that liver of Chrysolophus amherstiae and Aquila rapax are similar to that most birds.The hepatocytes arrangement of Chrysolophus amherstiae is regular and hepatic cord is a double and multiple rows arrangenged radially around a central vein.Interlobular artery,interlobular vein and interlobular bile duct in portal area are observed easily.The hepatic lobule of Aquila rapax are irregular and unclear.Bax protiein,c-Fos and TGF-β1 are expressed in cell membrane,nuclueus,cytoplasm and hepatic cord of hepatocyte in Chrysolophus amherstiae and Aquila rapax liver,but their expression intensities are different.Bax protein,c-Fos and TGF-β1 may have different functions,and their cooperative expression may control the growth,development of cells in bird liver.