电化学治疗对具多药耐药表型的人乳腺癌细胞MCF-7^(adr)的作用

Eeffects of electrochemical therapy in treating multidrug resistant human breast cancer cell line MCF- 7 ^(adr)

目的 :研究电化学治疗 (ECT)对具多药耐药表型的人乳腺癌细胞MCF 7adr的作用。方法 :以敏感型人乳腺癌细胞MCF 7为对照 ,采用细胞计数法观察ECT对乳腺癌耐药细胞株MCF 7adr的抑制效应 ,以Annex inV标记法观察ECT的诱导MCF 7adr细胞凋亡的作用。结果 :ECT对耐药细胞MCF 7adr的诱导凋亡及抑制效应接近于其对敏感细胞MCF 7的作用 ,无显著性差异 (P >0 0 5 )。结论

Purpose:To study the effects of electrochemical therapy (ECT) on treating multidrug resistant human breast cancer cell line MCF 7 adr . Methods:Using cell count assay,the inhibiting effect of ECT on adriamycin (ADR) resistant MCF 7 adr cells was observed, parental MCF 7 serving as control. Apoptosis in MCF 7 adr cells induced by ECT was assessed by AnnexinV affinity assay. Results:The effects of ECT inducing apoptosis and inhibiting proliferation on MCF 7 adr cells approximated to its effects on MCF 7 cells ( P >0 05). Conclusions:ECT is effective in treating multidrug resistant MCF 7 adr cells.