白细胞介素-1受体拮抗剂对NZB/WF_1小鼠狼疮肺损害防治作用的研究

Effect of interleukin-1 receptor antagonist on the prophylaxis and treatment of lung lesions in SLE

目的 探讨白细胞介素 1受体拮抗剂 (IL 1ra)在狼疮性肺损害防治中的价值。方法对有狼疮倾向的NZB/WF1小鼠腹腔内注射生理盐水和IL 1ra,对比观察其对细胞因子活性和肺部病理形态学改变的影响。结果 实验组和对照组IL 2活性分别为 (2 3± 4)U/ml和 (18± 3)U/ml(P <0 .0 5 ) ;NK细胞活性分别为 (76± 2 1) %和 (45± 4) % (P <0 .0 5 ) ;IL 1α分别为 (173± 78)pg/ml和 (44 2±10 0 )pg/ml(P <0 .0 1)。对照组小鼠肺组织病理变化重 ,表现为细支气管及小血管周围重度慢性炎性细胞浸润 ,毛细血管扩张充血 ,肺泡间质明显增厚及纤维化 ,而实验组小鼠毛细支气管和小血管周围炎性细胞浸润、毛细血管扩张 ,以及肺泡间质增厚均较对照组明显为轻。结论 IL 1ra在预防和治疗系统性红斑狼疮所致的肺损害方面有较好的效果。

Objective To evaluate the effect of interleukin 1 receptor antagonist(IL 1ra) on the prophylaxis and treatment of SLE induced lung damages. Methods Twelve New Zealand black /white F1 lupus prone mice were divided randomly into 2 groups. Normal saline 0.1 ml in control mice and IL 1ra 100μg (0.1 ml) in experiment mice, respectively were injected intraperitonealy once every other week for 3 times totally. The activity of cytokines and the lung pathology were observed. Results (1) The activity of cytokines showed IL 2 was (23±4) and (18±3) U/ml ( P <0.05), nature killing cell was (76±21) and (45±4)% ( P < 0.05), and IL 1α level was (173±78) and (442±100) pg/ml( P <0.01) in the experiment and control groups, respectively. (2) The lung pathology showed that infiltration of inflammatory cells around bronchium and vasculature, dilatation of blood capillary, and interstitial thickening and fibrosis in 5 of 6 control mice, and 4 of 6 experiment mice. The changes of lung pathology were obviously mild in experiment group compared with control group. Conclusion The preventive and treating effects of IL 1ra on SLE induced lung lesions were remarkable in alleviating the pathological changes. IL 1ra showed better effects on the prophylaxis and treatment of SLE induced lung damage.