东方田鼠抗日本血吸虫病CD74基因的差异表达及生物信息学分析

Different Expression and Bioinformatic Analysis of Microtus Fortis in Anti- Schistosomiasis Japonica CD74 Gene

利用日本血吸虫尾蚴感染东方田鼠,提取感染前和感染后10d和15d东方田鼠肝脏组织总RNA;利用大鼠CD74基因探针,经Rorthern杂交分析东方田鼠感染日本血吸虫前及感染血吸虫10 d、15 d后肝脏组织CD74的差异表达情况;同时,采用生物信息学分析大鼠CD74基因的cDNA序列及其编码的氨基酸序列以及CD74蛋白的结构域.结果显示:东方田鼠感染日本血吸虫感染血吸虫10 d、15 d后肝脏组织CD74的表达水平比感染前显著升高;大鼠CD74基因的cDNA序列全长1220bp,编码216个氨基酸残基;大鼠CD74蛋白含一个MHC2相互作用超家族结构域和一个MHCassoc_trimer超家族结构域.

The total RNA was extracted from microtus fortis liver tissue which was infected of the schistosoma japonicum cercariae before infection and after infection 10d and 15d. Using rattus norvegicus CD74 gene probe to hybridize analysis of CD74 difference expression in the microtus fortis liver tissues which were infected with Schistosoma japonicum before infection and after infection 10 d, 15 d. At the same time, bioinformatics analysis of the eDNA sequence and encoded amino acid sequence of the rattus norvegicus CD74 gene and CD74 protein structural domains. The results showed： after microtus fortis infected with schistosoma japonicum 10 d and 15 d the liver tissue of CD74 expression levels significantly higher than pre-infection; rattus norvegicus CD74 eDNA sequence of a total length is 1220bp and encode 216 amino acid residues; rattus norvegicus CD74 protein containing a MHC2 interaction domain of the superfamily and a MHCassoc trimer superfamily domain.