膀胱移行细胞癌中c-erbB-2、p53及p16蛋白的表达

p16, p53 and c- erbB-2 gene expression in bladder carcinoma

目的 探讨膀胱癌组织中p16、c erbB 2和p5 3蛋白表达与膀胱癌病理分级、临床分期和转移的关系。方法 应用免疫组化SP法对 75例膀胱癌组织中p16、p5 3及c erbB 2蛋白表达进行检测。结果 75例膀胱癌中p16、p5 3及c erbB 2的阳性率分别为 41.3% (31 75 )、44 .0 % (33 75 )和40 0 % (30 75 ) ,p16和c erbB 2蛋白在膀胱癌中的阳性率与肿瘤病理分级和临床分期有显著意义 (P<0 0 5 ) ,p5 3和c erbB 2阳性率与肿瘤临床分期及转移有密切的关系 (P <0 .0 1)。 77.3% (5 8 75 )肿瘤有上述蛋白的阳性表达 ,其中 5 3.3% (40 75 )的肿瘤同时有多个蛋白的阳性表达。结论 肿瘤的多因素分析比单因素分析更有价值 ,癌基因c erbB 2和抑癌基因p5 3。

Objective To investigate the expression of p16, p53 and c erbB 2 proteins in bladder carcinoma and to analyze their correlation with tumor grade, clinic stage and metastasis. Methods Immunohistochemical method was used to detect the p16, p53 and c erbB 2 gene expressions in 75 cases of bladder carcinoma. Results Positive rates of p16, p53 and c erbB 2 in 75 cases of bladder cancer were 41.3%, 44.0% and 40.0%, respectively. p16 and c erbB 2 gene expressions were related to the tumor grade and clinic stage ( P <0 05); p53 and c erbB 2 gene expressions were related to the clinic stage and metastasis ( P <0.01). Oncogenes and/or antioncogenes altered expressions were found in 77.3% cases of bladder carcinoma, and oncogenes and/or antioncogenes with multiple altered expressions were found in 53.3% cases. Conclusion The analysis of multiple oncogene and/or antioncogene is more valuable than that of single oncogene and/or antioncogene. The altered expression of oncogene c erbB 2 and antioncogenes p16 and p53 might play a promotive role in the tumorigenesis and development of bladder tumors.