缺氧耐受形成中各脑区氨基酸含量的变化

Chang of amino acid in generation tolerance to hypoxia

目的 探讨缺氧耐受形成机制。方法 高压液相法测定缺氧耐受形成中小鼠各脑区 L-天门冬氨酸(L - ASP)、L -谷氨酸 (L - GL U )、甘氨酸 (GL Y)、γ-氨基丁酸 (γ- GABA)含量变化。结果 L - ASP、L - GL U含量 :在端脑 ,1次缺氧组与正常对照组比二者均有升高趋势 ,3、4次缺氧组与 1次缺氧组比有下降趋势。在脑干、海马 ,各组之间无统计学差异。在小脑 ,3、4次缺氧组二者与正常对照组比均有明显降低。在间脑 ,随着缺氧次数的增加 L-ASP逐渐下降 ,4次缺氧组与 1次缺氧组及正常对照组比明显降低。各脑区 L - GL Y含量均随着缺氧次数的增多而增高 ,端脑、间脑的含量在缺氧 4次组有明显回降。各脑区 γ- GABA含量在缺氧耐受形成中均未见明显变化。结论 γ- GABA与缺氧耐受形成无明显关系 ;部分脑区 L - ASP、L - GL U的减少及 GL Y的回降促进了缺血耐受的形成。

Objective To explore mechanism of tolerance exposed to hypoxia. Method Detected L GLU,L ASP,GLY and γ GABA of different brain area in mouse with HPLC in generation of tolerance exposed to hypoxia. Result Contents of L ASP and L GLU in telencephalon tissue in run 1 group were higher than those in control group (P>0.05),contents of L ASP and L GLU in run 3,4 group were lower than those in run 1 group (P>0.05). In the brain stem tissues and hippocampal tissues,contents of L ASP and L GLU did not changs markerly in 4 groups. In the cerebellum tissues contents of L ASP and L GLU in runs 3 and 4 group were lower than those control group (P<0.05). In the diencephalon,contents of L ASP decreased with increasing hypoxic runs and contents of L ASP in runs 4 hypoxia group were significantly lower than those in control group and run 1 group (P<0.05). The concentration of GLY increased gradually with increasing hypoxia runs,but contents of GLY after four runs exposure reduced in telencephalon and diencephalon.Contents of γ GABA did not significantly change during repetitive exposure to hypoxia. Conclusions γ GABA is not markedly related to hypoxic tolerance,decreasing of L GLU,L ASP and reducing of L GLY enhance generation of tolerance to hypoxia.