摘要
目的 :探讨以颌下腺为靶器官 ,进行血管内皮生长因子 (VEGF)基因治疗后 ,颌下腺中VEGF含量变化。方法 :构建了VEGF基因表达的真核细胞表达载体 ,以阳离子脂质体为介质 ,向大鼠颌下腺转染外源性的VEGF基因 ,应用RT PCR技术观察VEGF基因的表达 ,ELISA方法检测颌下腺中VEGF的含量变化。结果 :转pBKCMV VEGF121基因后第1天 ,大鼠颌下腺内VEGF的表达及涎液中VEGF的含量即开始增高 ,术后2～3d达到高峰 ,之后逐渐下降 ,但高水平表达持续5d左右 ,与同期对照组相比 ,二者差别显著。结论 :以颌下腺为靶器官 ,阳离子脂质体为载体进行VEGF基因转染 ,可使外源基因在颌下腺内稳定持续表达 ,改变了涎液中细胞因子的含量 ,为临床上促进口内伤口的愈合提供了新的途径和依据。
Objective: To study the feasibility of using vascular endothelial growth factor (VEGF) gene therapy for salivary glands and observe the changes in content of VEGF in spittle. Methods: VEGF cDNA was cloned into eukaryotic expression vector pBKCMV and the VEGF gene was transferred into rat maxillary glands mediated by lipotect AMINE. The expression of VEGF gene was investigated by RT PCR and the content of VEGF in spittle was observed by ELISA. Results: The expression of VEGF gene and the content of VEGF were significantly higher in the experimental group than in the control. It reached its peak from the 2nd to 3rd d after treatment and remained high for 5 d. Conclusion: After being mediated by lipotect AMINE, the VEGF gene can express steadily and constantly. The content of VEGF in spittle becomes higher. It provides a new way for promoting oral wound healing in clinical practice.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第2期166-169,共4页
Journal of Third Military Medical University
关键词
颌下腺
基因治疗
大鼠
VEGF
CDNA
salivary gland
vascular endothelial growth factor
gene therapy
