GC-MS法测定芜菁子中油脂组成研究

Determination of oil Compositions in Brassica rapa L. seed by Gas Chromatography-Mass

目的:分析测定芜菁子中油脂的组成。方法:采用索氏提取法提取芜菁子中的油脂,甲酯化后,气相色谱-质谱法分析其油脂中脂肪酸的成分。结果:芜菁子中含有油酸,亚油酸,亚麻酸,硬脂酸,软脂酸,芥酸6种脂肪酸,其含量分别为12.5%、19.6%、8.9%、7.4%、5.8%、18.2%。结论:该法操作简便,灵敏度高,准确可靠,可用于芜菁子油中脂肪酸的组成及含量测定。

Objective： To determine the oil compositions and contents in Brassica rapa L. seed. Meth- ods： the oil of Brassica rapa L. seed was extracted by Soxhlet extraction method, and its component were analyzed by GC--MS, Results： there were six kinds of fatty acid in Brassica rapa L. seed. Its contents were oleic acid（12.5%）,linoleic acid（19.6%）,linolenic acid（8.9%） ,stearic acid（7.4%）,palmitic acid（5. 8%） ,erucic acid （18.2%）. Conclusions： The method was simple, sensitive, the results was accurate and reliable and can be used in the composition of fatty acids and determination in Brassica rapa L. seed.