沙利度胺对顺铂诱发大鼠异食癖的影响

Effect of thalidomide on cisplatin- induced pica in rats

目的观察沙利度胺对顺铂引起的大鼠异食癖的作用，探讨其抗化疗呕吐的实验基础。方法健康大鼠24只，随机分为对照组、模型组、低剂量沙利度胺组、高剂量沙利度胺组，观察72h内大鼠摄取高岭土所占总进食量的比值；另取大鼠48只，分组同前，分别于实验开始后5、33h，取大鼠延髓及胃窦组织，放免法测定P物质，免疫组化法测定神经激肽1受体（NK-1R）。结果顺铂10mg／kg可以引起大鼠异食癖，沙利度胺10mg／kg在顺铂应用24h后可以减轻这种异食癖（P〈0．05），同时伴有延髓及胃窦P物质的减少（P〈0．05），而NK-1R未出现明显变化。结论沙利度胺可以减轻顺铂引起的大鼠异食癖，提示在抗呕吐治疗中沙利度胺可能有一定的应用前景。

Objective To observe the effect of thalidomide on cisplatin - induced pica in rats, and to explore the ex- perimental basis of thalidomide resistant to chemotherapy - induced nausea and vomiting. Methods 24 healthy rats were randomly devided into 4 groups： control group, model group, thalidomide group （low dose） and thalidomide group （high dose）. The ratio of intake of kaolin total food intake within 72 h was observed. Another 48 rats were randomly derided into 4 groups which were the same as stated above, medullar tissue and gastric antrum were taken out at 5, 33 hours after the start of the experiment, the substance P （SP） were measured by radioimmunoassay and the neurokinin 1 receptor （NK - 1R） determined by immunohistochemistry method. Results Cisplatin 10 mg/kg induced pica,which could be decreased by thalidomide at 10 mg/kg 24 h after the application of cisplatin （P 〈 0.05 ）, while accompanied with reduced SP level （P 〈 0.05）, but NK - 1R did not have significant changes. Conclusion Thalidomide can reduce cisplatin - induced pica in rats, which suggests that thalidomide may have some prospect in the treatment of anti - chemotherapy vomiting.