红树林放线菌筛选及其抗肿瘤活性测定

Screening of Mangrove Forest Actinomycetes and Its Antitumor Activity Detection

目的从我国湛江红树林采集的海泥样品中分离纯化微生物菌株并进行筛选及抗肿瘤活性测定。方法采用形态学方法鉴定放线菌菌株;采用四氮唑盐(MTT)法测定筛选出的72株放线菌发酵液对肺癌细胞A549与人类慢性髓性白血病细胞K562两种肿瘤细胞的细胞毒活性。结果经鉴定分离得到了72株放线菌,其中18%的放线菌发酵液具有不同程度的细胞毒活性。N2010-37和N2010-68两株放线菌发酵液对上述两种肿瘤细胞株作用较显著。结论该研究结果为从湛江红树林放线菌中寻找抗肿瘤活性成分奠定了基础。

Objective To isolate and purify the microbial strains from sea mud samples collected in Zhanjiang mangrove wetland for screening and detecting the antitumor activity, Methods The morphological method was adopted to identify actinomycetes strains. The MTT assay was applied to measure two tumor cells lines （A549 and K562） cytotoxicity of 72 strains of actinomycete fermentation broth. Results By identification of isolated 72 strains of actinomycete,18 percents of actinomycete fermentation broth showed the cytotoxicity in varying degrees. Especially,the fermentation broth of N2010-37 and N2010-68 revealed obvious antitumor activity on the above- mentioned two tumor cells lines. Conclusion The research results estabhsh the foundation for seeking the antitumor components from mangrove forest actinomycete in Zhanjiang.