摘要
目的测定安神补心片中丹参酮ⅡA和丹酚酸B的含量。方法采用Diamonsil C18柱(250 mm×4.6 mm,5μm),梯度洗脱,流动相A为乙腈、B为水、C为甲醇、柱温35℃,检测波长为270 nm。结果丹参酮ⅡA进样量在0.013 21~0.264 2μg范围内,丹酚酸B进样量在0.061 10~1.222 0μg范围内与峰面积均有较好的线性关系,加样回收率丹参酮ⅡA为98.60%,丹酚酸B为98.58%。结论该方法结果准确、快速,可作为安神补心片中丹参酮ⅡA和丹酚酸B的含量测定方法。
Objective To develop a RP- HPLC assay for the determination of tanshinone IIA and salvianolic acid B in Anshen Buxin Tablets. Methods The assay carried out on Diamonsil C18 column(250 mm ×4.6 mm, 5 um) with gradient elution. The mobile phase A was acetonitrile and the mobile phase B was water and the mobile phase C was methyl alcohol. The temperature of chromatographic column was 35℃ at a 270 nm detection. Results The standard curve was linear in the range of 0. 013 21 ug- 0. 264 2 ug for Tanshinone IIA and 0.06 110 ug - 1. 222 0 ug for salvianolic acid B. The mean recovery was 98.60% and 98.58%, respectivly. Conclusion This method is accurate and reproducible for the determination of Tanshinone HA and salvianolic acid B in Anshen Buxin Tablets..
出处
《中国药业》
CAS
2012年第14期46-47,共2页
China Pharmaceuticals