西洋参DNA导入大豆实现遗传转化的研究Ⅱ.导入后代异黄酮类成分的含量测定

Genetic Transformation of Soybeans by Soaking Its Seeds with the Total DNA of American Ginseng (Panax quinquefolium) Ⅱ. Determination of Isoflavone Content by HPLC

用高效液相色谱法测定了西洋参 DNA导入大豆后代的异黄酮类成分、大豆苷 (daidzin)、大豆黄素(daidzein)、染料木苷 (genistin)、染料木素 (genistein)的含量 ,采用 U nisil Q C1 8柱 ,4.6 mm× 2 5 0 m m,流动相乙腈 - 0 .0 0 3mol/L 磷酸氢二钠 (17∶ 83) ,流速 1.0 m L/min,检测波长 2 5 4nm,结果表明 ,4种成分分离效果好 ,成分含量发生了广泛变异 。

A method for the determination of isoflavone content in the transformed progenies of soybean by HPLC was reported. Using unisil Q C 18 column (4.6 mm×250 mm, 5 μm), with a mobile phase of CH 3CN 0.003 mol/L Na 2HPO 4 (17∶83), a detecting wavelength at 254 nm, and flow rate of 1 0 mL/min, it was found that the contents of daidzein, genistein, daidzin and genistin in the transformed progenies of soybean changed greatly and could be separated effectively, which makes it possible to cultivate and develop the best progeny for further development.