艾纳香二氢黄酮对脂质过氧化损伤大鼠原代培养肝细胞的保护作用

Protective Actions of Blumea Flavanones on Primary Cultured Hepatocytes against Lipid Peroxidation

研究 5种艾纳香二氢黄酮类化合物对过氧化损伤的原代培养肝细胞的保护作用。胶原酶灌流法分离大鼠肝细胞。以 CCl4或 Fe SO4+Cys损伤原代培养肝细胞 ,1× 10 - 4 和 1× 10 - 5 mol/ L 的 5种艾纳香二氢黄酮均能明显抑制受损伤细胞的转氨酶逸出、MDA产生及 GSH耗竭。其中以 2 ,5 -二羟基艾纳香二氢黄酮的作用最强 ,在 CCl4所致肝细胞损伤实验中 ,其抑制 MDA产生的 ED5 0 约为 2 .6× 10 - 6 mol/ L ,保护 GSH的 ED5 0 约为 1.0 8× 10 - 5 mol/ L ,抑制 AL T逸出细胞的 ED5 0 约为 9.39× 10 - 6 mol/

To search for the protective actions of blumea flavanones (BFs) on hepatocytes against lipid peroxidation, rats' hepatocytes were isolated and cultured with or without blumea flavanones, then demaged by FeSO 4 +cysteine or CCl 4. The lipid peroxidation criteria (malondialdehyde production and GSH depletion) and alteration in hepatocyte membrane (leakage of GPT and GOT) were estimated. It was found that BFs at 10 and 100 μmol/L inhibited the MDA generation, GSH depletion and GPT leakage of hepatocytes induced by CCl 4 or FeSO 4+cysteine. Among BFs, BF Ⅱ possessed the strongest activity.