硒对氟致大鼠血清 肾和股骨中铜 锌 铁影响的研究

Study on the Effects of Selenite on the Changes of Copper, Zinc and Iron in Serum,Kidney and Femur Induced by Fluoride in Rats

给大鼠饮用含氟化钠 ( 1 50 mg/kg)及同时分别加入不同浓度亚硒酸钠 ( 0 .5～ 2 .0和 4.0mg/L)的饮水共 1 0周 ,观察氟及氟与硒联合作用对大鼠血清氟、尿氟和股骨氟的影响 ,以及大鼠血清 ,肾组织和股骨中铜、锌、铁含量的变化。结果表明 ,氟暴露可使血清、尿和股骨氟水平显著升高及血清、肾、股骨的铜、锌、铁代谢紊乱。同时给予 2 .0 mg/L亚硒酸钠可使血清氟和尿氟进一步升高 ,而股骨氟含量降低 ,并对氟致血清、肾组织和股骨中铜、锌、铁的代谢紊乱具有显著调整作用 ,但 0 .5和 4.0 mg/L亚硒酸钠的这种作用较弱。可以认为 ,亚硒酸钠对尿氟排泄具有显著促进作用 ,对氟诱导的微量元素代谢紊乱具有明显拮抗作用 ,2 .0 mg/L是拮抗1 50 mg/L氟化钠引起的微量元素代谢紊乱的最佳浓度。

Five groups of SD male rats were provided with deionized drinking water containing o and 150 mg/L NaF,and containing both 150 mg/L NaFand 0.5,2.0 or 4.0 mg/L Na 2SeO 3 respectively for 10 weeks,in order to observe influences of fluoride and combined respectively for 10 weeks,in order to observe influences of fluoride and combined effects of selenite and fluoride on the contents of fluorine ion in serum,urine and femur of rats,as well as changes of the contents of copper,zinc and iron in serum,kidney and femur.the results showed that fluoride could cause the significant increase of levels in serum,urine and femur and metabolic disorders of copper,zinc or iron in serum,kidney and femur of rats.Simultaneous providing of 2.0 mg/L Na 2SeO 3 and fluoride in drinking water could incur more obvious increases of fluorine in serum and urine,and decrease of fluorine level in femur,as well as the significant antagonistic action on metabolic disturbances of copper,zinc and iron in serum,kidney and femur of rats,whereas those of 0.5 or 4.0 mg/L Na 2SeO 3 were not significant.It is concluded that selenite possesses significant acceleration of fluoride excretion in urine and antagonistic effect of metabolic disturbances of trace elements induced by fluoride and 2.0 mg/L Na 2SeO 3 is the optimal concentration for the antatgonistic effects on the metabolic disorder of trace elements induced by 150 mg/L NaF in drinking water.