流动分析法测定烟草中的纤维素

Determination of Cellulose in Tobacco with Flow Analyzer

建立了测定烟草中纤维素含量的流动分析法。采用纤维素酶酶解烟草中的纤维素,利用流动分析仪测定水解液中的葡萄糖。结果表明:①最优酶解条件为:烟粉0.25 g,缓冲溶液60 mL,酶用量0.25 g,水解温度37℃,水解时间24 h;②方法的相对标准偏差(RSD)为2.40%,回收率为96.7%～103.8%,检测限为0.11 mg/mL。该方法适合于烟草中纤维素含量的测定。

A flow analysis method for determining the cellulose in tobacco was developed. Cellulose was hydrolyzed by cellulase and the formed glucose was determined by flow analyzer, The results showed that： 1） The optimal hydrolysis conditions were： tobacco powder 0.25 g, buffer solution 60 mL, enzyme 0.25 g, hydrolysis temperature 37℃,hydrolysis time 24 hours. 2）The relative standard deviation （RSD） of the method was 2.40% with the recoveries from 96.7% to 103.8% and the limit of detection of 0.11 mg/mL. This method is suitable for determining the content of cellulose in tobacco,