响应面优化酶催化米糠油甘油解制备甘油二酯

Optimization of diacylglycerol production from lipase-catalyzed glycerolysis of rice bran oil by response surface methodology

利用响应面(RSM)对叔丁醇溶剂体系中的脂肪酶Novozym 435催化米糠油甘油解反应合成甘油二酯(DAG)的反应条件进行了优化。在单因素实验基础上选取反应温度、反应时间、酶添加量(酶和米糠油质量比)、底物质量比(米糠油:甘油)等4个因素作为自变量,以DAG的产率为响应值,进行5水平4因素中心组合旋转设计(CCRD)优化,确定了影响DAG合成的关键因素以及最佳反应条件。分析结果表明,在各影响因素中,底物质量比对DAG的产率影响最大。综合考虑优化和节约后,利用模型计算DAG合成的最佳条件:反应温度60℃,反应时间10.5h,加酶量10.66%,底物质量比16,在此条件下DAG产率是53.08%。

Response surface methodology （RSM） was applied to optimize the diacylglycerol （DAG） production from Novozym 435-catalyzed glycerolysis of rice bran oil in t-Butanol system. Based on single-factor analysis, 5-1evel-4-factor central composite rotatable design（CCRD） was employed to evaluate and optimize the effect of DAG synthesis parameters,such as reaction temperature,time,enzyme amount（mass ratio of lipase to rice bran oil）,and substrate mass ratio （rice bran oil：glycerol）. The results indicated that substrate mass ratio was the most important variable. Considering the economical efficiency as well as optimization,the optimum DAG synthesis conditions with the yield of 53.08% were as follows.reaction temperature 60℃,time 10.5h,enzyme amount 10.66%,substrate mass ratio 16.