竹黄无性型菌的人工培养研究

Artificial culture of the anamorph of Shiraia bambusicola

采用培养特征观察,竹红菌甲素测定和分子生物学等技术和方法,研究了不同培养基上竹黄菌的生物量,产竹红菌素甲素和形成子座情况,结果表明,葡萄糖20g,琼脂20g,竹汁1000mL(竹枝200g煮沸取滤液),pH自然的培养基中并插入灭菌的竹枝,接入竹黄菌种,7d后,该培养基上菌丝生物量和其竹红菌素量均为最高,且约6个月室内室温(0～20℃)培养获得了类天然竹黄状的培养物,该培养物经过了竹红菌甲素测定及分子鉴定分析多途径的综合验证。通过本实验,竹黄无性型菌的人工培养取得一定进展。

The biomass,hypocrellin A and forming fruit body of Shiraia bambusicola on the different medias were explored. Through the method of culture characters,hypocrellin A determination and molecular biology,the results showed that mycielia biomass and hypocrellin A were the best on the medium consisting of glucose 20g, agar 20g, bamboo filter liquor（bamboo pole 200g boiled） 1000mL, and inserted sterile bumboo poles ; And on above medium,S, bambusicola-like fruit bodies were obtained when the strain were inoculated and cultivated under the room temperature （0-20℃） lasting six months. S. bambusicola-like fruit bodies were confirmed by hypocrellin A determination and molecular identification. The experiments showed that the fruit body of S. bambusicola on artificial culture made progress.