响应面法优化豆渣DNA提取中的细胞裂解工艺

Optimization of cell lysis conditions of soybean dregs in the process of DNA extraction by response surface methodology

采用响应面法对豆渣DNA提取中的细胞裂解工艺进行优化。在单因素实验基础上,选取SDS(十二烷基硫酸钠)浓度、裂解液pH、裂解温度为影响因子,利用Box-Behnken中心组合进行三因素三水平的实验设计,以DNA提取率为响应值,进行响应面分析。结果表明,裂解时间70min时,豆渣细胞裂解的最优条件为:SDS浓度1.84%、裂解液pH5.95、裂解温度81℃。在此条件下,DNA提取率的理论值为0.63%,实测值为0.625%。

Response surface methodology （RSM） was used for optimizing the cell lysis conditions of soybean dregs in the process of DNA extraction. Based on single-factor experiment,three independent variables, namely the concentration of Sodium dodecy sulfate（SDS）,pH and lysis temperature,were selected as affecting factors during lysis. The experiment was arranged according to Box-Behnken central composite design. The results showed that the optimum lysis conditions for soybean dregs were as follows.concentration of SDS was 1.84% ,pH was 5.95,1ysis temperature was 81℃ when lysis time was 70min. The predicted DNA extraction yield was 0.63% ,while the actual extraction yield was 0.625%.