摘要
以人促凋亡蛋白基因bax全长转录本cDNA为模板,经PCR扩增得到bax基因,将其克隆到pMDl8-T,并构建原核表达质粒载体pGEX-4T-1-Bax,转化大肠杆菌感受态细胞BL21(DE3),IPTG诱导目的蛋白表达。PCR扩增、双酶切鉴定和测序结果均显示bax基因已被成功克隆到表达载体中,表达产物经SDS-PAGE检测,证实为GST-Bax融合蛋白。
Using eDNA as template, bax gene was cloned into pMD18-T. The expression vector pGEX-4T-1-Bax was then constructed and transformed into E. coli BL21（DE3）. The expression of target protein was induced by IPTG. According to PCR, enzyme digestion and sequencing, bax gene was successfully cloned into the expression vector. SDS-PAGE test of the product showed that GST-Bax was expressed.
出处
《湖北农业科学》
北大核心
2012年第13期2858-2861,共4页
Hubei Agricultural Sciences
关键词
BAX
克隆
原核表达
bax
cloning
prokaryotie expression
