摘要
本试验主要研究沙棘果榨汁后的沉淀物中黄酮的最佳提取工艺。采用乙醇回流浸提的方法提取沙棘果泥中的黄酮,使用分光光度计对沙棘果泥中的黄酮含量进行测定。利用正交试验考察提取温度、提取时间以及料液比对黄酮提取率的影响,最终确定提取黄酮的最佳工艺。结果表明:最佳提取条件为提取温度75℃、提取时间2h、料液比1∶45。
The major objective of this experiment is to optimize the extraction technology of flavonoids from Hippophae rhamnoides puree after juicing. Prior to determination of the flavonoids content by spectrophotometer, ethanol refluxing was employed to isolate flavonoids. Orthogonal experiments were adopted to analyze the effect of each factor in extraction temperature, extraction time and solid- liquid ratio on extraction percentage of flavonoids to ascertain the optimized extraction technology.
关键词
沙棘果泥
黄酮
乙醇回流浸提
Hippophae rhamnoides puree
flavonoids
ethanol refluxing
