PEO/PVA水凝胶伤口敷料的毒性和疗效评价

TOXICITY AND HEALING EFFECT OF PEO/PVA HYDROGELS AS WOUND DRESSINGS

为了评价经冻融循环处理和电子束辐射交联接技技术合成的聚氧化乙烯与聚乙烯醇（PEO／PVA）混合物水凝胶伤口敷料的性能；研究了该水凝胶膜时机体和细胞的毒性以及对皮肤伤口的愈合作用。毒性作用通过急性全身毒性试验、细胞毒性试验和皮肤斑贴试验检测。细胞毒性试验应用高敏感性的成纤维细胞持续培养7d的RPMI－1640培养系统，检测细胞生长抑制和镜下细胞形态学变化。结果显示，PEO／PVA水凝胶敷料没有引起机体任何急性毒性反应以及皮肤刺激与致敏反应；细胞增殖抑制指数和镜下细胞形态与正常对照细胞无任何差异。伤口愈合试验是将水凝胶敷料覆盖于大鼠背部烧伤处，与医用纱布敷料处理比较，伤口愈合时间明显缩短（p＜0．01）；更换敷料容易，即更换敷料时对新生上皮和肉芽组织无损伤破坏作用；没有敷料残余物滞留于伤口。结果证实，PEO／PVA混合物水凝胶伤口敷料具有良好的生物相容性，并且该水凝胶膜可有效地加速伤口愈合。

Toxicity and healing effect of poly(ethylene oxide) (PEO)/poly(vinyl alcohol) (PVA) blend hydrogel films as wound dressings were evaluated. Toxicity testing is checked by acute general toxicity, in vitro cytotoxicity and sticking on skin assay. We used a highly sensitive 7 d RPMI-1640 cell culture with fibroblasts as cytotoxicity test system. Cytotoxicity is assessed by monitoring cell growth inhibition, supported by cell morphological evaluation using light dricroscopy (LM). It was shown that PEO/PVA blend hydrogels did not induce any acute general toxic effects on mice and an allergic reaction for Iabbit skin. The cell growth inhibition and morphology at the LM level were the very same as those of the control culture. To evaluate the healing effect of the blend hydrogels on burn, the hydrogels covered a wound formed on the back of rats. As compared with the corresponding gauze dressing group, the healing under the wet environment of the hydrogel dressing had some advantages: the wound healing is faster(p < 0.01).it is easier to change the dressing without any damage to the regenerated surface and no dressing material remains on the wound. It is concluded that PEO/PVA blend hydrogel films may be considered as truly biocompatible, and the wet environment formed by thc hydrogels is effective for fast healing of wound.