环氧化酶2和Ki-67在口腔鳞状细胞癌中的表达及相关性研究

Cydooxygenase-2 and Ki.67 expression in the development of oral squamous cell carcinoma

目的研究Ki-67和环氧化物酶2（COX-2）在口腔鳞状细胞癌中的表达及相互关系。方法采用免疫组化方法检测28例口腔扁平苔藓组织（15例单纯扁平苔藓、13例伴异常增生）、28例口腔鳞状细胞癌组织及15例正常口腔黏膜组织中Ki-67、COX-2的表达。结果COX-2在正常口腔黏膜组织中不表达、Ki-67组织仅为低表达或不表达；口腔鳞状细胞癌组、单纯增生组和异常增生组COX-2和Ki-67的高表达率明显高于正常口腔黏膜组[COX-2分别为64．3％（18／28）、33．3％（5／15）、30．8％（4／13）比0（0），67．9％（19／28）、26．7％（4／15）、30．8％（4／13）比0（0），均P〈0．01]，口腔鳞状细胞癌组COX-2和Ki-67的高表达率明显高于单纯增生组和异常增生组（均P〈0．01）。口腔鳞状细胞癌中COX-2的表达阳性率为85．7％（24／28），Ki-67为85．7％（24／28）；二者表达呈正相关（r=0．8，P〈0．05）。结论Ki-67和COX-2在口腔鳞状细胞癌发生、发展中起着重作用，二者表达呈正相关，在口腔鳞癌的发生发展中有协同作用。

Objective To investigate the expression of Ki-67 and Cyclooxygenase-2（COX-2） in oral squamous cell carcinoma. Methods The expression of Ki-67 and COX-2 in 28 OLPs tissues, 28 oral squamous cell carcinoma tissues （including 15 well differentiated cases, 13 moderately differentiated cases ） and 15 normal oral tissues was analyzed by immunihisto chemical method. Results Expression of Ki-67 and COX-2 in simple lichen planus group and the dysplasia group tissues was significantly higher than that in the normal oral tissues [ 33.3% （5/ 15）, 30.8% （4/13） vs 0（0） ;26.7% （4/15）, 30.8% （4/13） vs 0（0）, all P 〈0.01 ]. Expression of Ki-67 and COX-2 in oral squamous cell carcinoma tissues was significantly higher than that in the OLPs tissures （P 〈 0.01 ）. A significant positive correlation was found between Ki-67 and COX-2 in oral squamous cell carcinoma. Conclusion Oral squamous cell carcinoma is a continuous and stepwise course; Ki-67 and COX-2 may play an important role in the course.