花蟹壳红色素提取工艺优化

Optimization of extracting technology for red pigment from Portunus pelagicus shell

分别以二氯甲烷、石油醚、乙醇和水为提取剂浸提花蟹壳中红色素,其中乙醇浸提花蟹壳红色素效果好,并在475 nm处有最大吸收峰。选用乙醇为提取剂,以浸提液在475 nm处的吸光值为指标,分别研究浸提温度、粉碎粒度、提取时间、料液比和乙醇浓度对花蟹壳红色素提取结果影响。在单因素研究基础上,以85.5%乙醇为提取剂,提取时间为90 min时进行浸提温度、粉碎粒度和料液比L9(33)正交实验,确定浸提温度70℃,花蟹壳粉碎粒度90目,料液比为1∶10(w/v)所得浸提液在475 nm处吸光值最高。

Red pigments were extracted from Portunus pelagicus shell with methylene chloride, petroleum ether, ethanol and water as extractant respectively. The ethanol displayed good extracting activity for the red pigment, and the maximum absorbance was observed at 475 nm. Therefore, ethanol was selected as the suitable extractant, and the absorbance of the leaching liquor at 475 nm was used as the indicator. The influencing factors for the red pigment extraction including extracting temperature, granularity, ex- tracting time, ratio of material to liquid and ethanol concentration were studied. The orthogonal experiment of L9 （3^3） was performed to optimize the extracting temperature, granularity and ratio of material to liquid, based on the results of single factor experiment with 85.5 % of ethanol as extractant and 90 min as extracting time. The result showed that the optimal parameters were extracting temperature 70 ℃ the granularity 90 eyelet, and the ratio of material to liquid 1：10 （w/v）, in this case the absorbance of the leaching liquor at 475 nm reached the top.