高碘对大鼠下腔静脉内皮细胞活化的影响

Effects of Excessive Intake of Iodine on Activation of Endothelial Cells of Inferior Vena Cava in Rats

目的研究高碘对大鼠下腔静脉肝段内皮细胞活化的影响。方法将32只断乳约1个月的远交封闭群SPF/VAF级Wistar大鼠随机分为4组,分别为正常对照(自来水)组、10倍碘组、50倍碘组和100倍碘组,每组8只,雌雄各半。采用自由饮水、摄食的方式进行染毒,各组大鼠碘摄入量分别为6.15、61.5、307.5、615μg/d,连续染毒6个月。测定大鼠血浆细胞间黏附因子-1(ICAM-1)、血管性血友病因子(vWF)及血管性血友病因子前肽(vWFpp)含量。结果与正常对照组相比,50倍碘组雄性大鼠血浆vWF含量显著升高,100倍碘组雄性和雌性大鼠血浆ICAM-1、vWF含量均显著升高;且随着摄入碘含量的升高,雄性和雌性大鼠血浆ICAM-1、vWF含量呈上升趋势。而各组雄性和雌性大鼠血浆vWFpp含量均未见显著变化。结论高碘可导致大鼠内皮细胞活化,并在一定程度上呈剂量-效应关系。

Objective To observe the effect of excessive iodine on the activation of endothelial cells of inferior vena cava in rats. Methods A total of 32 outhreeding closed colony Wistar rats, about one month after stopping lactation, were randomly divided into four groups：normal control group （tap-water）; 10- fold excessive Iodine group （10HI）; 50- fold excessive Iodine group（5OHl）; 100- fold excessive Iodine group（100HI）,with four males and four females in each group. The rats were treated with iodine through food at dosage of 6.15, 61.5, 307.5 and 615 p^g/d, respectively. After 60 days of exposure,venous blood was collected and ICAM-1, vWF, vWFpp were determined. Results Compared with normal control group,vWF increased in 50HI male group, ICAM-land vWF significantly increased in 100HI male and female group, vWFpp showed no significant changes in each group. The endothelial cells appeared morphological change under optical microscope. Conclusion Excessive iodine may induce endothelial cell activation with dose-dependent manner.