摘要
目的:探讨经鼻腔给予外源性转化生长因子β1(transforming growth factor-β1,TGF-β1)对大鼠癫痫持续状态(statusepilepticus,SE)后血脑屏障(blood brain barrier,BBB)通透性的影响。方法:建立匹罗卡品(pilocarpine)SE模型。SD大鼠252只随机分成正常对照组、模型组和TGF组,各组设计7个观察时间点,即SE后3 h、6 h、24 h、2 d、3 d、4 d、7 d。各大鼠在相应的时间点处死取材,应用化学定量的方法检测大鼠脑组织内伊文思蓝(Evans blue,EB)的含量,应用免疫组织化学法检测相应时间点海马区的zonula occluden-1(ZO-1)蛋白的表达。结果:大鼠脑内的EB含量与正常对照组相比,模型组和TGF组都先增加,24 h达到峰值,后逐渐下降,7 d接近正常;TGF组与模型组比较,脑内EB含量均低于模型组,6 h至2 d增加均有统计学意义(P<0.05~P<0.01)。TGF组和模型组比较,海马区ZO-1蛋白的表达都是先降后升,以24 h为最低,其中6 h、24 h、2 d差异均有统计学意义(P<0.05~P<0.01),TGF组明显高于模型组,低于正常对照组。结论:经鼻腔给予外源性TGF-β1能降低大鼠SE后BBB的通透性。
Objective: To investigate the effect of intranasal delivery of exogenous transforming growth factor-β1 (TGF-β1) on the permeability of blood brain barrier(BBB) after status epilepticus (SE) in Sprague-Dawley rats. Methods: SE model was induced by pilocarpine. Two hundred fifty-two SD rats were divided into normal control group, model group and TGF group randomly, each of which was reassigned into seven sub-groups:3 h, 6 h, 24 h, 2 d, 3 d, 4 d and 7 d groups after SE. The change or BBB permeability was determined by detecting Evans blue (EB) content in rat brains with chemic-quantitative analysis;The protein expression of zonula occluden-1 (ZO-1) in hippocampus was analyzed by immunochemistry. Results:The EB contents in model group and TGF group were significantly increased, compared with control group, in which the peak of EB was emerged at 24 h after SE, and then decreased gradually,closed to normal at 7 d;Compared with model group,EB contents in TGF group were decreased at 6 h to 2 d(P 〈0.05 to P 〈 0.01 ) with significant difference. In model and TGF groups, the protein expression of ZO-1 was firstly declined, and then gradually increased ;the lowest expressions were emerged at 24 h after SE;and there was significant differences of ZO-1 expression at 6 h ,24 h was 2 d points between the two groups(P 〈 0.05 to P 〈 0. 0.01). The expression of ZO-1 protein in TGF group was lower than control group. Conclusions:Intranasal delivery of exogenous TGF-β1 decreases BBB permeability after SE.
出处
《蚌埠医学院学报》
CAS
2012年第7期748-753,共6页
Journal of Bengbu Medical College
基金
国家自然科学基金资助项目(30970997)
关键词
癫痫持续状态
转化生长因子Β1
血脑屏障
大鼠
status epilepticus
transforming growth factor-βl
blood brain barrier
rats