水荭花药材中8个指标成分的UPLC-MS/MS法测定

Determination of Eight Marker Compounds in Flowers of Polygonum orientale by UPLC-MS/MS

建立了超高效液相色谱-串联质谱法同时测定水荭花药材中的没食子酸、原儿茶酸、儿茶素、花旗松素、山柰素-3-O--D-葡萄糖苷、槲皮素-3-O--L-鼠李糖苷、山柰素-3-O--L-鼠李糖苷和槲皮素。采用Acquity BEH C18色谱柱,流动相为0.1%甲酸乙腈溶液-0.1%甲酸,梯度洗脱。使用电喷雾离子化三重四级杆串联质谱仪,以多反应离子监测(MRM)方式检测。8个指标成分分别在0.8～25、0.1～4、0.9～28、3～96、0.3～10、0.6～18、0.8～25和0.3～8 g/ml浓度范围内线性关系良好,平均加样回收率为91.2%～109.3%,RSD为1.2%～7.9%。

An UPLC-MS/MS method was established for the determination of gallic acid, protocatechuic acid, catechin, taxifolin, kaempferol-3-O-β-D-glucoside, quercetin-3-O-α-L-rhamnoside, kaempferol-3-O-α-L-rhamnoside and quercetin in flowers ofPolygonum orientale. An Acquity BEH C18 column was used with the mobile phase of 0.1% formic acid aeetonitrile solution-0.1% formic acid solution by gradient elution. A TQD tandem mass spectrometry equipped with electrospray ionization source was used and operated by multiple reaction monitoring （MRM） ion mode. The calibration curves of the eight marker compounds were linear in the range of 0.8 - 25, 0.1 - 4, 0.9 - 28, 3 - 96, 0.3 - 10, 0.6 - 18, 0.8 - 25 and 0.3 - 8 ug/ml, resoectivelv. The recoveries were 91.2 % - 109.3 %, with RSDs of 1.2 % - 7.9 %.