豫南地区汉族人群类风湿关节炎患者IL-18基因启动子-607C/A多态性检测

Detection of IL-18 Gene Promoter-607 C/A Polymorphisms in Han Population with Rheumatoid Arthritis in the South of Henan Province

采用序列特异性引物的聚合酶链反应技术(PCR-SSP)分析110例类风湿关节炎(RA)患者和100例健康体检者的IL-18基因启动子在-607位点的单个核苷酸多态性,检测豫南地区汉族人群白细胞介素18(interleukin-18,IL-18)基因启动子单个核苷酸多态性与类风湿性关节炎(rheumatoid arthritis,RA)的发生是否存在关联.结果表明,RA患者组与健康对照组在IL-18基因-607位点的等位基因频率的分布及基因型频率的分布比较差异均有统计学意义(χ2=8.668、4.225,P<0.05),抗CCP抗体阳性组和阴性组在IL-18基因-607位点的基因型频率分布差异没有统计意义(χ2=0.941,P>0.05),说明IL-18基因启动子-607位点的多态性与RA患者类风湿因子存在关联.

Using polymerase chain reaction with sequence specific primers method （ PCR - SSP） , the single nu- cleotide polymorphisms of the promoter region of IL - 18 gene at position - 607 was detected in 110 RA patients and 100 controls. Results showed that there was significant difference in the distributions of the IL - 18 - 607 genotype frequencies and allele frequency in RA patients compared with those of controls （χ2=8. 668,4. 225 ,P 〈 0.05）. On the distribution of IL- 18 -607 genotypic frequencies, no significant difference was observed between positive group and negative group of the anti-CCP antibody （χ2 = 0. 941, P 〉 0. 05） and the polymorphism of IL - 18 gene at position - 607 might show correlation with RF in RA patients.