淫羊藿对激素性股骨头坏死大鼠血液流变学及骨密度的影响

Effect of epimedium on blood rheology and bone density of rats with steroid-induced femoral head necrosis

目的观察淫羊藿提取液对激素性股骨头坏死大鼠血液流变学及骨密度的影响。方法健康SD大鼠32只，雌雄各半，随机分为4组：空白组、模型组、淫羊藿高剂量组、淫羊藿低剂量组，每组8只。后3组采用臀肌注射醋酸泼尼松龙注射液制备激素性股骨头坏死模型，造模的同时淫羊藿高、低剂量组分别给予含生药浓度1．5、0．9g／L的淫羊藿提取液10ml／kg，模型组及空白组给予同等剂量的生理盐水，共6周。结果经灌胃6周后，淫羊藿高剂量组和低剂量组全血高切黏度为（5．55±0．60）、（5．85±0．66）ms／s；血浆黏度为（1．88±0．10）、（1．93±0．17）ms／s；高于空白组相应指标[（4．48±0．56）、（1．64±0．11）ms／s，P〈0．05]，淫羊藿高剂量组全血高切黏度、血浆黏度低于模型组相应指标[（6．66±0．65）、（2．11±0．21）ms／s，P〈0．05]。淫羊藿高剂量组全血低切黏度、红细胞压积分别为（9．96±0．68）、（0．48±O．05）ms／s，均低于模型组[（12．21±1．11）、（0．60±0．07）ms／s，P〈0．05]，且淫羊藿高剂量组低于淫羊藿低剂量组[（10．97±0．67）、（0．57±0．05）ms／s，P〈0．05]。淫羊藿高剂量组和空白组骨密度高于淫羊藿低剂量组和模型组，差异有统计学意义（P〈0．05）。结论高剂量淫羊藿提取液对激素性股骨头坏死的作用机制可能与降低血液流变学的各项指标、保证组织有效灌注、改善股骨头的局部微循环，并能够抑制破骨细胞活性、促进体外成骨细胞的增殖和分化成熟、增强骨质密度有关。

Objective To observe the effect of epimedium extract on blood rheology and bone den- sity of rats with steroid-induced femoral head necrosis. Methods Thirty-two healthy SD rats were selected, half of female and half of male, and randomly divided into four groups ： control group, model group, high- close epimedium group and low-dose epimedium group, 8 rats in each group. The steroid-induced femoral head necrosis models were established by gluteal injection of prednisolone acetate injection in last three groups. High-dose epimedium group and low-dose epimedium group were given the concentration of 1. 5 μL and 0.9 μL crude drugs containing epimedium extract 10 ml/kg respeetively, and model group and blank group were given the same dose of normal saline, 6 weeks. Results After gastric infusion for 6 weeks, the whole blood high shear viscosity in high-dose epimedium group and low-dose epimedium group was （5.55 ±0. 60） and （5.85 ±0. 66） ms/s, and the plasma viscosity was （ 1.88 ±0. 10） and （ 1.93 ± 0. 17 ） ms/s, respectively, which were significandy higher than those in control group （P 〈 0.05 ）. The whole blood high shear viscosity and plasma viscosity in high-dose epimedium group were lower than those in the model group [ （6.66 ±0. 65）, （2. 11 ±0. 21） ms/s, P 〈0.05]. The whole blood low shear viscos- ity and hematoerit in high-dose epimedium group were （9. 96 ± 0. 68 ） and （0. 48 ± 0.05 ） ms/s respective- ly, which were lower than those in model group （ P 〈 0. 05 ） and low-dose epimedium group [ （ 10.97 ± 0. 67） and （0. 57 ± 0. 05 ） ms/s, P 〈 0. 05 ） ]. The bone density in high-dose epimedium group and con- trol group was higher than that in low-dose epimedium group and model group （ P 〈 0. 05 ）. Conclusion The mechanism of high-dose epimedium extract for the treatment of steroid-induced femoral head necrosis may be related with many factors such as to reduce the blood rheology indicators, ensure the effective perfusion of organization and improve the local microcirculation of femoral head, as well as to inhibit the osteoclast activity, promote the osteoblast differentiation and maturation in vitro and enhance bone density.