生物活性玻璃修饰的聚对苯二甲酸乙二醇脂对成骨细胞增殖及活性的影响

Effect of polyethylene terephthalates coated with bioactive glass on osteoblastic proliferation and cy- toactivity in vitro

目的现在较为广泛应用于临床的人工韧带[聚对苯二甲酸乙二醇脂（PET）]——因为所固有的疏水性，使其在临床应用受到一定局限，本实验拟寻找促进材料与骨的相容的新方法。方法运用临床常用的涂层技术，以生物玻璃（58S）作为涂层材料作用于PET纤维表面，将成骨细胞（MC3T3-E1）培养在以涂层PET纤维和未涂层PET纤维上，观察成骨细胞的细胞增殖能力和细胞活性。结果细胞计数的第1、3、5天，细胞数量在涂层组与空白组均呈现增长趋势，但是在5d涂层处理组明显高于未处理组[（6．25±0．27）×104比（8．92±0．17）×104，P〈0．05]，而在噻唑蓝法（MTT）和碱性磷酸酶（ALP）活性测定中，涂层组在第1、3、5天均表现出明显的优势（P〈0．01）。结论经生物玻璃涂层处理的PET纤维组能显著提高成骨细胞的增殖及生物学活性。

Objective To search for a novel method to promote the compabibility of materials and bone. The anterior cruciate ligament （ACL） reconstruction using artificial ligament [ polyethylene tereph- thalates （PET）] , which has good biocompatibility and mechanical properties, is prevalent. However, the low tendon-to-bone healing rate due to the PET＇ s hydrophobicity limits the clinical application. Methods This study aimed to observe the effects of the coating bioactive glass （58S） synthetic anterior eruciate liga- ment （ACL） on osteoblastie proliferation and eytoaetivity in vitro. By using coating technique, bioaetive glass （58S） was coated on the polyethylene terephthalates （PET） fibers. The scanning electron micro- graphs showed that bioaetive glass （58S） were spindle-shaped and well-orientated on the PET fibers. Then, MC3T3-E1 osteoblasts were seeded onto the PET or the coating PET. Results The number of osteo- blasts was significantly increased in the coating PET （8.92 ± 0. 17 ） as compared with the PET （6. 25± 0. 27 ） by using automated cell counter at the 5th day （P 〈 0. 05 ）. Alkaline phosphatase activity and meth- yl thiazol tetrazolium （MTF） test also showed that the coating fibrous scaffold induced significnat osteogen- esis and cell proliferation as compared to PET without coating. Conclusion The coating bioaetive glass （58S） provides an instructive microenvironment for osteoblast proliferation.