三磷酸腺苷抑制肿瘤坏死因子-α诱导的肌源性干细胞凋亡及机制

Inhibitory effect of adenosine-triphosphate on tumor necrosis factor-a-induced apoptosis of muscle- derived stem ceils

目的探讨三磷酸腺苷（ATP）对肌源性干细胞（MDSCs）凋亡的抑制作用及其机制。方法采用差速贴壁法分离新生小鼠MDSCs，利用40μg/L肿瘤坏死因子（TNF）-α诱导MDSCs凋亡，100txmol／LATP抑制细胞凋亡。经流式细胞仪检测MDSCs的凋亡率和半胱氨酰天冬氨酸特异性蛋白酶（Caspase）-3／8的表达。结果TNF-α诱导后，脱壁细胞明显增多，MDSCs的凋亡率由5．17％升至31．58％，增加了5倍，Caspase-3染色阳性细胞率由5．01％增至19．25％，Caspase-8染色阳性细胞率由2．97％增至8．58％。ATP作用后，MDSCs的凋亡率降至12．06％，减少了2／3，Caspase．3染色阳性细胞率降至9．86％，Caspase-8染色阳性细胞率降至6．96％，但仍高于对照组。结论ATP能抑制MDSCs表达Caspase-3／8，减轻TNF-α诱导的MDSCs凋亡。

Objective To explore the inhibitory effects of adenosine-triphosphate （ATP） on apop- tosis of muscle-derived stem cells （ MDSCs ） and the mechanism. Methods MDSCs were obtained from mouse skeletal muscle by preplate technique. The apoptosis of MDSCs was induced by 40 μg/L tumor necrosis factor （TNF）-α, and inhibited by 100 μmo]/L ATP. The apoptosis rate and the expression of Caspase-3/8 were measured by flow eytometer. Results As compared with that in group A, the apoptosis rate （31.58%） and the expression level of Caspase-3/8 proteins （19. 25%/8.58% ） were increased markedly in group C. As compared with that in group C, the apoptosis rate （12. 06% ） and ratio of Caspase-3 ＋/8＋ MDSCs （9. 86%/6. 96% ） were decreased significantly in group D. Conclusion ATP could inhibit the expression of Caspase-3 and Caspase-8, which may reduce the TNF-α-induced apoptosis of MDSCs.