小肠RNA对小鼠电离辐射肠道损伤修复的差异基因表达的研究

DIFFERENTIALLY EXPRESSED GENES IN REPAIRING IRRADIATION-INDUCED DAMAGE IN MOUSE INTESTINAL TRACT BY FOREIGN MOUSE SMALL INTESTINAL RNA

为从基因水平探讨小肠核糖核酸，促进小鼠电离辐射肠道损伤修复的机理，采用随机分组法把 ９０只小鼠分成 ４组，建立辐射后给予 ４０μｇ ＲＮＡ治疗的实验组与 ０．４ｍＬ生理盐水治疗的对照组模型，分别于照射后６、１２、２４ｈ，４ｄ和８ｄ采集小肠空肠段标本，运用消减杂交基础上的ＬＤ－ＰＣＲ技术，分离实验组与对照组之间的差异表达基因。结果表明；实验组与对照组６、 １２、 ２４ｈ， ４ｄ和 ８ｄ的消减后 ＬＤ－ＰＣＲ产物克隆成功，共获取 １６５个新克隆，其中实验组６、 １２、 ２４ｈ， ４ｄ和 ８ｄ的克隆新表达基因数分别为 １８、 ２２、 ２５、 １３和 １２个，编号依次为 ＸＣＺ１－９０，即为 ９０个与注入ＲＮＡ修复密切相关的高表达基因；对照组 ６、 １２、 ２４ｈ；４ｄ和 ８ｄ 的克隆新表达基因数分别为 ２２、 ２５、 １４、 ６和 ８个，编号为 ＡＸＣＺ１－７５，即为 ７５个可能与电离辐射损伤相关的高表达基因。本工作成功地建立了辐射后给予ＲＮＡ治疗的实验组与生理盐水治疗的对照组模型；从实验组与对照组之间分离并克隆了１６５个新基因，其中９０个与注入 ＲＮＡ修复密切相关的高表达基因。 ７５个可能与电离辐射损伤相关的高表达基因。

To study the differentially expressed genes correlative to irradiation-induced damage in mouse intestinal tract and its repair by foreign mouse small intestinal RNA, Divide 90 mice into 4 groups randomly. For the experimental groups, each mouse was given 40μg foreign mouse small intestinal RNA, and for the control groups, 0.4mL of 0.9% NaCI. The intestinal specimens were collected form each group 6h, 12h, 24h, 4d and 8d after 60Co γ-rays irradiation respectively. The differentially expressed genes in the tested and control groups were checked and cloned by long-distance PCR based on subtractive hybridization. 165 differential gene clones were obtained, of which 75 named as AXCZ1-75 maybe were correlated with the irradiation-induced damage in mouse intestinal tract; and 90 named as XCZ1-90 maybe were correlated with repairing of the damaged mouse intestinal tract by foreign mouse small intestinal RNA,