大鼠放射性皮肤溃疡组织中c-Fos、Rb蛋白表达研究

A PATHOLOGICAL STUDY ON EXPRESSION OF c-Fos AND Rb PROTEINS IN RAT RADIATION SKIN ULCER

为研究大鼠放射性皮肤溃疡组织中 ｃ－Ｆｏｓ、 Ｒｂ表达。采用 １４０只 Ｗｉｓｔａｒ大鼠进行局部照射（７射线）制备放射性皮肤溃疡动物模型。观察病变 １年，然后采用 ｃ－Ｆｏｓ、 Ｒｂ多克隆抗体及免疫组化方法检测皮肤溃疡组织中 ｃ－Ｆｏｓ、 Ｒｂ的表达情况。结果表明；ｃ－Ｆｏｓ、 Ｒｂ的表达阳性率分别为４５．８％（３３／７）、 ６３．９％（４６／７２，两者阳性部位主要见于增生肥大的鳞状上皮细胞胞核、增生的间质成纤维细胞胞质及胞核、小血管内皮细胞中。初步的结论是；ｃ－Ｆｏｓ、Ｒｂ蛋白的高表达可能与放射性皮肤溃疡难愈合有关。

To study the expression of c-Fos and Rb in radiation-induced rat skin ulcers. We set up an animal model of radiation skin ulcer with 140 rats which were locally irradiated with 35-55Gy rays, and the pathological changes were observed for 1 year. Immunohistochemical studies were performed in 72 rat radiation skin ulcer tissues, and c-Fos, Rb protein polyclonal antibodies were used. The results showed that the positive rate for overexpression of c-Fos was 45.8%, and for that of Rb was 63.9%. The positive position was mainly in the nucleus of activated squamous epithelial cells and in some fibroblasts, endotheliocytes of the arterioles in mesenchyme. It is suggested that the overexpression of c-Fos and Rb proteins might be related to canceration and poor healing in radiation skin ulcers.